General method of inoculation into liquid media of the test sample and decimal dilutions, incubation at 30°C for 24 h or 48 h, calculation of the number of coliforms per gram or millilitre of sample (i.e. the MPN) from the number of tubes in which gas has formed, using a table for determining the most probable number.
BS 5763-3:1979
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Methods for microbiological examination of food and animal feeding stuffs-Enumeration of coliforms – most probable number technique at 30°C
BSI Group , 10/31/1979
